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. 2022 Nov 2;611(7936):614–622. doi: 10.1038/s41586-022-05386-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Coverage of genes, euchromatic TEs and heterochromatic TEs (left Y axis; 500 kb windows) and H2B.8 enrichment in sperm (right Y axis; 1 kb windows) along Chromosomes 2 to 5. Chromosome 1 is shown in Fig. 4d. b, Genome snapshots of H2B.8 abundance (log₂(IP/input)) and transcription (log₂(RPKM)) in p35S::H2B.8-eGFP seedlings, and gene and TE annotations (orange, euchromatic TE; magenta, heterochromatic TE) over representative regions. RPKM, Reads Per Kilobase of transcript per Million mapped reads. c, Profiles and associated heatmaps of H2B.8 enrichment in p35S::H2B.8-eGFP seedlings over genes (grouped by seedling expression), TEs (grouped by chromatin state) and intergenic regions. d, Proportions (%) of the genome covered by genes, TEs and intergenic regions in wild-type sperm and seedling, and by respective H2B.8 peaks in pH2B.8::H2B.8-eGFP h2b.8 sperm and p35S::H2B.8-eGFP seedling. Same color coding is used as in (c) and Fig. 4b. e, Upper panel, scatterplot showing anticorrelation of H2B.8 enrichment with GC content (%) in p35S::H2B.8-eGFP seedlings over denoted genomic features. Lower panel, scatterplot showing the WT seedling anti-GFP enrichment (log₂(IP/input)) control against GC content (%). ρ, Spearman’s Rank. f, Prediction of H2B.8 using indicated chromatin features, transcription (log₁₀(RPKM+1)) and GC content as predictors. The y-axis shows the adjusted R² value between the predicted and observed values. g, Profiles of H2B.8 enrichment in p35S::H2B.8-eGFP seedlings over non-genic (TE and intergenic) regions. The test regions were grouped into three equal parts by GC content levels (low GC < 0.29; 0.29 < middle GC < 0.34; high GC > 0.34).