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. 2022 Nov 2;611(7936):614–622. doi: 10.1038/s41586-022-05386-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Sperm nuclear sizes in WT and other indicated genotypes. P values calculated using one-sided ANOVA followed by individual two-sample Tukey tests. Boxplots marked as A and B are significantly different between groups (P < 0.001) but not within the group (P > 0.1). n = 80 (WT, h2b.8-1, pH2B.8::H2B.8–Myc h2b.8-1, pH2B.8::H2B.8-N234K–Myc h2b.8-1), 79 (pH2B.8::H2B.8–eGFP h2b.8-1, h2b.8-2) or 39 (pH2B.8::H2B.8ΔIDR–eGFP h2b.8-1) nuclei examined over two independent experiments. b,c, Super-resolution 3D-SIM images (left) and associated intensity profiles (right) of sperm and seedling nuclei of indicated genotypes (h2b.8 refers to h2b.8-1 unless specified otherwise). Data shown represent three independent experiments. Scale bars, 1 μm (b), 1 μm (c, upper two panels) and 2 μm (c, lower three panels). d, Root nuclear sizes in indicated genotypes (numbers after the genotype indicate independent transgenic lines). P values calculated using one-sided ANOVA followed by individual two-sample Tukey tests. Boxplots marked as A and B are significantly different between groups (P < 0.05) but not within the group (P > 0.1). n = 100 (WT), 101 (p35S::H2B.2–YFP-9), 103 (p35S::H2B.8–eGFP-3, p35S::H2B.8ΔIDR–YFP-5), 104 (p35S::H2B.8–eGFP-5), 107 (p35S::H2B.8ΔIDR–YFP-2) or 109 (p35S::H2B.2–YFP-8) nuclei examined over two independent experiments.