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. 2022 Nov 2;611(7936):614–622. doi: 10.1038/s41586-022-05386-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Western blot showing H3K9me2 levels in leaf and sperm nuclei, with histone H3 as a control. Data shown represent two independent experiments. Rep, biological replicate. For source data, see Supplementary Fig. 1. b, Peptide counts from sperm (SN), vegetative (VN) and leaf nuclei (LN) mass spectrometry. cH2B, canonical H2B. c, Confocal images of H2B.8 (pH2B.8::H2B.8-eGFP) incorporation during male gametogenesis. Lowest panel is a duplicate of Fig. 1d. MN, VN, GN and SN, respectively, microspore, vegetative, generative and sperm nucleus. Data shown represent three independent experiments. Scale bars, 5 μm. d, Confocal images of various tissues from pH2B.8::H2B.8-eGFP plants. Data shown represent three independent experiments. Scale bars, 20 μm (early embryo, leaf, and root) and 5 μm (seed).