Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 2;611(7936):614–622. doi: 10.1038/s41586-022-05386-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 2 — a, Alignment of h2b.8 CRISPR lines. h2b.8-1 has a single base deletion at 76 bp as indicated by an arrow, leading to a premature stop codon after 33 amino acids. h2b.8-2 has a 12 bp deletion after 67 bp and another of 5 bp after 92 bp, producing a truncated 54 amino acid protein. b, Nuclear sizes of the generative nucleus and sperm nuclei of indicated genotypes. P-values, one-sided ANOVA followed by individual two-sample Tukey tests, which adjust for multiple comparisons. Boxplots marked as A, B and C are significantly different between groups (P < 0.001) but not within the group (P > 0.1). n = 30 (generative nucleus), 80 (WT, h2b.8-1), and 77 (h2b.8-2) nuclei examined over two independent experiments. c, Density plot of individual SYBR Green-stained voxel intensities from wild-type (WT, blue) and h2b.8 (red) sperm nuclei. n = 30 nuclei for each. d, e, Boxplots indicating the volumes (d) and intensity (e) of SYBR Green foci in WT (blue) and h2b.8 (red) sperm. P-value, independent two-sample t-test. n = 30 nuclei for each. AU, arbitrary unit. f, g, Colocalization of DAPI and H2B.8-eGFP signals in sperm (f, pH2B.8::H2B.8-eGFP) and in H2B.8 ectopically expressed seedlings (g, p35S::H2B.8-eGFP). n = 37 and 31 nuclei for sperm and seedlings, respectively. h, Confocal images and quantification of H2B.8 condensates in p35S::H2B.8-eGFP seedlings. P-value, independent two-sample t-test. n = 30 and 71 for nuclei with small and large H2B.8 condensates, respectively, examined over two independent experiments. Scale bars, 2 μm.