Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 2;611(7936):614–622. doi: 10.1038/s41586-022-05386-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a, Alignment of Arabidopsis H2B variants (N-terminal tail – upper panel, C-terminal body – lower panel). The N-terminal tail IDR of H2B.8 is highlighted with a yellow dashed box. Amino acids are colored according to the RasMol scheme. b, Intrinsic disorder prediction of H2B.8 (red) and H2B.2, a canonical H2B (blue), by PONDR. Histone profiles are aligned at the interchange between tail and body domains (dashed line). H2B.8 N-terminal tail IDR is highlighted in yellow. c, SDS-PAGE of reconstituted histone octamers containing H2B.2, H2B.8, H2B.8ΔIDR or H2B.8-scrambledIDR. For gel source data, see Supplementary Fig. 1. d, Fluorescence recovery after photobleaching (FRAP) of H2B.2- or H2B.8-containing chromatin droplets. Average relative fluorescence intensity of the photobleached area across 6 individual chromatin droplets was calculated and displayed at each time point. Error bars, ±SD. Scale bars, 1 μm. e, Fusion of H2B.2- or H2B.8-containing chromatin droplets (histone H3 and dsDNA were labeled with AF594 and YOYO-1, respectively) over time. Data shown represent one representative experiment, which has been performed twice with similar results. Scale bars, 1 μm. f, g, In vitro phase separation of nucleosomal arrays (NA) containing H2B.2, H2B.8 or H2B.8ΔIDR at indicated salt and NA concentrations (in g, NA concentration for all panels was 50 nM). Histone H3 and dsDNA were labeled with AF594 and YOYO-1, respectively, and merged images of red and green channels are shown. Data shown represent one representative experiment, which has been performed three times with similar results. Scale bars, 2 μm. h, Nuclear size quantification of Nicotiana benthamiana epidermis cell nuclei transiently expressing native and chimeric H2B.8 as indicated. Boxplots marked as A and B are significantly different between groups (P < 0.001) but not within the group (P > 0.1). n = 30 (WT, p35S::H2B.8-EWSR1-IDR-eGFP, and p35S::H2B.8-TAF15-IDR-eGFP), 32 (p35S::H2B.8-eGFP), and 33 (p35S::H2B.8-scrambledIDR-eGFP) nuclei from two independent experiments.