TABLE 1.
Bacterial strains, cosmids, and plasmids used in this study
| Strain, cosmid, or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | F−endA hsdR17 (rk− mk+) supE44 thi-1 recA1 gyr-A96 φ80lacZ | 23 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F′ proAB lacIZ ΔM15 Tn10) | Stratagene |
| MC1061 | (λpir), thi thr-1 leu6 proA2 his-4 argE2 lacY1 galK2 ara14 xyl5 supE44, λpir | 43 |
| SM10 | (λpir), thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Kmr, λ pir | 43 |
| A. hydrophila | ||
| AH-3 | Wild type, serogroup O:34 | 36 |
| AH-405 | Rifampin-resistant mutant derived from AH-3 | 39 |
| AH-3PLA1 | pla insertion AH-405 mutant obtained with pFS-PLA | This work |
| AH-3PLA4 | pla insertion AH-405 mutant obtained with pFS-PLA | This work |
| AH-3PLC2 | plc insertion AH-405 mutant obtained with pFS-PLC | This work |
| AH-3PLC3 | plc insertion AH-405 mutant obtained with pFS-PLC | This work |
| Cosmids | ||
| pLA2917 | Tcr Kmr | 1 |
| COS-PLA | pLA2917 with 20-kb chromosomal AH-3 Sau3A insert (pla gene) | This work |
| COS-PLC | pLA2917 with 20-kb chromosomal AH-3 Sau3A insert (plc gene) | This work |
| Plasmids | ||
| pBR328 | Apr Cmr Tcr | 44 |
| pBR-PLA1 | pBR328 with 8.3-kb HindIII insert from COS-PLA (pla gene) | This work |
| pBR-PLA2 | HpaI-EcoRV 4.5-kb insert of pla gene derived from pBR-PLA1 | This work |
| pBR-PLC | pBR328 with 3-kb HindIII insert from COS-PLC (plc gene) | This work |
| pBluescript SK | Aprori of ColE1 | Stratagene |
| pSK-PLA | pBluescript SK with insert of pBR-PLA2 (pla gene) | This work |
| pSK-PLC | pBluescript SK with insert of pBR-PLC (plc gene) | This work |
| pACYC184 | Cmr Tcr | 44 |
| pACYC-PLA | pACYC184 with insert of pBR-PLA2 (pla gene) | This work |
| pACYC-PLC | pACYC184 with insert of pBR-PLC (plc gene) | This work |
| pFS100 | pGP704 suicide plasmid, λpir dependent, Kmr | 43 |
| pFS-PLA | pFS100 with an internal fragment (1,230 bp) of pla | This work |
| pFS-PLC | pFS100 with an internal fragment (697 bp) of plc | This work |