TABLE 3.
Determination of some extracellular and periplasmic activities in different E. coli and A. hydrophila strains as described in Materials and Methods
| Strain | Cellular fractiona | Hemolysis on erythrocytes of:
|
Cytotoxicity on:
|
|||
|---|---|---|---|---|---|---|
| Sheep | Bovine | Rainbow trout | Vero cells | EPC cells | ||
| E. coli | ||||||
| DH5α | S | —b | — | — | — | — |
| P | — | — | — | — | — | |
| DH5α + pACYC-PLA | S | — | — | — | — | — |
| P | — | — | — | — | — | |
| DH5α + pACYC-PLC | S | — | — | 1/8 | 1/4 | 1/16 |
| P | — | — | 1/16 | 1/64 | 1/128 | |
| A. hydrophila | ||||||
| AH-405 (AH-3 Rifr) | S | 1/64 | 1/64 | 1/128 | 1/64 | 1/128 |
| AH-3PLA1; pla insertion mutant from AH-405 | S | 1/64 | 1/64 | 1/128 | 1/64 | 1/128 |
| AH-3PLA4; pla insertion mutant from AH-405 | S | 1/64 | 1/64 | 1/128 | 1/64 | 1/128 |
| AH-3PLA1(pACYC-PLA); mutant pla complemented | S | 1/64 | 1/64 | 1/128 | 1/64 | 1/128 |
| AH-3PLA4(pACYC-PLA); mutant pla complemented | S | 1/64 | 1/64 | 1/128 | 1/64 | 1/128 |
| AH-3PLC2; plc insertion mutant from AH-405 | S | 1/64 | 1/64 | 1/128 | 1/4 | 1/16 |
| AH-3PLC3; plc insertion mutant from AH-405 | S | 1/64 | 1/64 | 1/128 | 1/4 | 1/16 |
| AH-3PLC2(pACYC-PLC); mutant plc complemented | S | 1/64 | 1/64 | 1/128 | 1/32 | 1/64 |
| AH-3PLC3(pACYC-PLC); mutant plc complemented | S | 1/64 | 1/64 | 1/128 | 1/32 | 1/128 |
a
S, supernatant; P, periplasmic fraction.
b
—, not detected.