TABLE 4.
Virulence for rainbow trout and mice of A. hydrophila AH-3 (serogroup O:34), defined insertion mutants, and mutants complemented by pla or plc
| Strain and main characteristics | LD50a for:
|
|
|---|---|---|
| Rainbow trout | Mice | |
| AH-3; wild-type | 05.3 | 107.4 |
| AH-405; AH-3 rifampin-resistant mutant | 105.4 | 107.6 |
| AH-3PLA1; pla insertion mutant from AH-405 | 105.6 | 107.7 |
| AH-3PLA4; pla insertion mutant from AH-405 | 105.5 | 107.5 |
| AH-3PLA1(pACYC-PLA); mutant pla complemented | 105.7 | 107.5 |
| AH-3PLA4(pACYC-PLA); mutant pla complemented | 105.5 | 107.6 |
| AH-3PLC2; plc insertion mutant from AH-405 | 107.4 | >109.0 |
| AH-3PLC3; plc insertion mutant from AH-405 | 107.3 | >109.0 |
| AH-3PLC2(pACYC-PLC); mutant plc complemented | 105.6 | 107.7 |
| AH-3PLC3(pACYC-PLC); mutant plc complemented | 105.7 | 107.6 |
a
The values are the averages of three independent experiments, and the maximum deviation was always <100.4.