Table 1.
Overview of the ABPP-identified enzymes from profiling the supernatant of P. chrysosporium suspension cultures grown on minimal medium supplemented with beech wood chips.
| Protein ID | Annotation | Used ABP | Log2 fold change | MW [kDa] | Predicted function |
|---|---|---|---|---|---|
| Phchr2|126075 | CE1-CBM1 | FP-alkyne | 7.19 | 35.59 | acetyl-xylan esterase |
| Phchr2|2912243 | CE15 | FP-alkyne | 5.35 | 44.29 | 4-O-methyl-glucuronoyl methylesterase |
| Phchr2|2915237 | GH5 subfamily 9 | JJB111 | 10.71 | 46.55 | 1,4-β-endoglucanase |
| Phchr2|3024052 | GH3 | JJB111 | 8.55 | 86.85 | 1,4-β-xylosidase |
| Q66NB7; Phchr2|2985730 | GH5 subfamily 6 | JJB111 | 8.45 | 40.41 | 1,4-β-glucanase |
| Q9URP5;O74203 | CBM1-GH3 | JJB111 | 6.82 | 85.56 | β-glucosidase |
| Phchr2|2895579 | GH5 subfamily 9 | JJB111 | 5.59 | 73.50 | 1,3-β-glucosidase |
| Phchr2|2990154 | GH30 subfamily 3 | JJB111 | 5.58 | 65.13 | 1,6-β-endoglucanase |
| Phchr2|3002168 | DUF | JJB111 | 5.39 | 74.43 | DUF1793, DUF4964, DUF4965, DUF5127 |
| Phchr2|3004260 | GH79 | JJB111 | 4.99 | 48.99 | β-glucuronidase |
| Phchr2|2981757 | GH5 subfamily 5 | JJB111 | 3.26 | 42.05 | 1,4-β-endoglucanase |
| Phchr2|2927412 | GH74 | JJB111 | 2.99 | 79.15 | exo-1,3-β-xyloglucanase/reducing end-specific cellobiohydrolase |
| Phchr2|2916357 | GH28 | JJB111 | 2.52 | 53.53 | galacturonase |
| W5ZNX7;C6H06; Phchr2|123909 | GH16 | JJB111 | 2.21 | 33.57 | endo-1,3-β-glucanase |
| Phchr2|3006243 | GH18, CBM5 | JJB111 | 2.09 | 54.31 | endo-1,3-β-glucanase |
Two SHs, 12 GHs, and one protein (DUF) with four DUFs with similarities to GHs were elucidated from FP-alkyne and JJB111 labeling, respectively.