Table 2.

Overview of the ABPP-identified enzymes from profiling the substrate-bound fraction (SBF) of P. chrysosporium suspension cultures grown on minimal medium supplemented with beech wood chips.

Protein ID	Annotation	Used ABP	Log2 fold change	MW [kDa]	Predicted function
Phchr2|3002242	GH3	JJB111	6.20	92.86	β-glucosidase
Phchr2|3004009	GH17	JJB111	4.73	33.78	1,3-β- glucanosyltransferase
Phchr2|2895579	GH5	JJB111	4.44	73.50	1,3-β-glucosidase
Phchr2|2915237a	GH5, subfamily 9	JJB111	4.11	46.55	1,4-β- endoglucanase
Phchr2|2945552	GH3-WSC	JJB111	3.99	98.95	β-glucosidase
Phchr2|3038646	GH25	JJB111	3.75	24.47	muramidase
Phchr2|3003144	GH3	JJB111	2.65	92.83	β-glucosidase
Phchr2|2912243a	CE15	FP-alkyne	6.35	44.29	glucuronoyl methylesterase
Phchr2|2983171	CE1, CBM1	FP-alkyne	5.93	31.85	acetyl-xylan esterase
Phchr2|2918304	CE15, CBM1	FP-alkyne	3.24	47.47	glucuronoyl methylesterase
Phchr2|126075a	CE1, CBM1	FP-alkyne	2.37	35.59	acetyl-xylan esterase

^aEnzymes that were also labeled in the supernatant (Table 1).

Four SHs and seven GHs were elucidated from FP-alkyne and JJB111 labeling, respectively.