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. 2022 Nov 16;12:19694. doi: 10.1038/s41598-022-24055-2

Table 2.

Summary of findings of meta-analyses of the effect of riceland mosquito control on Anopheles larval density (the number of larvae and pupae per dip or area sampler), arranged by the type of control, study design and geographical region.

Study Country Predominant vector Details of intervention (application method, rate, dose, frequency, timing, fish species) Study design Plot size (no. of replications*) Relative percent difference (95% CI)
Larviciding
Surface agents
 Reiter (1980) Kenya An. gambiae s.l Monomolecular surface film (lecithin solution) at rate of 2.47 L/ha CTS1 600 m2 (9) − 60.0 (− 74.0, − 38.5)
 Reiter (1980) Kenya An. gambiae s.l Monomolecular surface film (lecithin solution) at rate of 4.94 L/ha CTS 600 m2 (15) − 57.1 (− 76.3, − 22.3)
 Bukhari et al. (2011) Kenya An. gambiae s.l Monomolecular surface film (Aquatain, silicone-based) at 1 ml/m2 (1st application) and at 2 ml/m2 (2nd application) CTS 2000 m2 (6) − 29.1 (− 79.0, + 138.7)
RE model for all studies − 57.2 (− 69.4, − 40.3)
 Karanja et al. (1994) Kenya An. arabiensis Monomolecular surface film (Arosurf MSF) at 4 L/ha every 14 days CITS2 100 m2 (4) − 91.6 (− 99.9, + 486.3)
Synthetic organic chemicals
 Allen et al. (2008) USA An. quadrimaculatus Lambda-cyhalothrin, aerial application at 5.5 g AI/ha, once (1 ×) prior permanent flooding CTS 13–15 ha (2) − 9.3 (− 40.9, + 39.0)
 Ravoahangimalala et al. (1994) Madagascar An. gambiae s.s Deltamethrin emulsionable concentrate 25 5 g/ha, 1 × CTS 58–110 m2 (2) − 92.7 (− 95.4, − 88.5)
 Ravoahangimalala et al. (1994) Madagascar An. gambiae s.s Deltamethrin emulsionable concentrate 25 12.5 g/ha, 1 × CTS 43–58 m2 (2) − 92.9 (− 96.5, − 85.8)
 Yap and Ho (1977) Malaysia Anopheles spp. Chlorpyrifos (Dursban) at 14 gm/ha, 1 × CTS (3) − 79.0 (− 91.8, − 46.5)
 Yap and Ho (1977) Malaysia Anopheles spp. Chlorpyrifos (Dursban) at 28 gm/ha, 1 × CTS (3) − 75.2 (− 90.6, − 34.5)
 Yap and Ho 1977) Malaysia Anopheles spp. Chlorpyrifos (Dursban) at 56 gm/ha, 1 × CTS (3) − 67.8 (− 82.3, − 41.4)
 Yap and Ho (1977) Malaysia Anopheles spp. Organophosphorus (Dowco-214) at 56 gm/ha, 1 × CTS (3) − 68.0 (− 83.6, − 37.5)
 Yap et al. (1982) Malaysia Anopheles spp. Temephos (Abate 500E) 60 gm/ha, 1 × CTS 69–365 m2 (2) − 56.3 (− 86.8, + 45.0)
 Yap et al. (1982) Malaysia Anopheles spp. Temephos (Abate 500E) 100 gm/ha, 1 × CTS 69–365 m2 (2) − 77.0 (− 93.0, − 24.4)
 Yap et al. (1982) Malaysia Anopheles spp. Temephos (Abate 500E) 200 gm/ha, 1 × CTS 69–365 m2 (2) − 61.2 (− 89.5, + 43.2)
 Teng et al. (2005) Taiwan An. sinensis Temephos (Abate 1-SG) at 1 ppm, 2 × (20 day interval) CTS 119–194 m2 (4) − 91.2 (− 97.5, − 69.3)
RE model for all studies − 73.1 (− 83.8, − 55.4)
 Kamel et al. (1972) Egypt An. pharoensis Iodofenphos (NUVANOL N20U), aerial application at 1.5 L/ha, 1 × CITS 50–120 ha (2) − 93.2 (− 98.1, − 76.2)
 Kamel et al. (1972) Egypt An. pharoensis Iodofenphos (NUVANOL N20U), aerial application at 3 L/ha, 1 × CITS 50–120 ha (2) − 50.2 (− 83.3, + 49.0)
RE model for all studies − 72.3 (− 89.5, − 26.9)
Biological larvicides
 Allen et al. (2008) USA An. quadrimaculatus Bacillus thuringiensis var. israelensis (Bti), AQUABACxt, aerial application at 108 L/ha on a 61-m swath, 1 × CTS 13–15 ha (3) − 60.8 (− 86.9, + 17.1)
 Dennett et al. (2001) USA An. quadrimaculatus Bacillus sphaericus (Bs), VectoLex WDG, aerial application at 1.68 kg/ha, 1 × CTS 2000 m2 (2) − 8.6 (− 24.1, + 10.1)
 Dennett et al. (2001) USA An. quadrimaculatus Bs, VectoLex WDG, aerial application at 0.56 kg/ha, 1 × CTS 2000 m2 (2) − 11.1 (− 24.2, + 4.2)
 Ravoahangimalala et al. (1994) Madagascar An. gambiae s.s Bti, Teknar HP-D liquid concentrate, at 0.6 L/ha, 1 × CTS 58–68 m2 (2) − 81.1 (− 86.1, − 74.4)
 Ravoahangimalala et al. (1994) Madagascar An. gambiae s.s Bti, Teknar HP-D liquid concentrate, at 1.25 L/ha, 1 × CTS 58–78 m2 (2) − 87.7 (− 92.7, − 79.5)
 Ravoahangimalala et al. (1994) Madagascar An. gambiae s.s Bti, Teknar HP-D liquid concentrate, at 12.5 L/ha, 1 × CTS 58–87 m2 (2) − 93.2 (− 96.1, − 88.0)
 Sundaraj and Reuben (1991) India An. subpictus Bs, Biocide-S 1593 M, at 2.2 kg/ha, 1 × after transplantation CTS 440 m2 (3) − 74.9 (− 90.5, − 33.5)
 Sundaraj and Reuben (1991) India An. subpictus Bs, Biocide-S 1593 M, at 4.3 kg/ha, 1 × after transplantation CTS 440 m2 (3) − 75.8 (− 92.4, − 22.7)
 Kramer et al. (1988) USA An. freeborni Bti, Vectobac (200 ITU/mg), at 6 kg/ha, 2 × (when mosquito densities were high) CTS 1000 m2 (3) − 56.1 (− 81.3, + 2.8)
 Teng et al. (2005) Taiwan An. sinensis Bti, Vectobac G, at 1 g/m2, 2 × (20 day interval) CTS 119–194 m2 (4) − 83.8 (− 94.9, − 48.6)
 Teng et al. (2005) Taiwan An. sinensis Lagenidium giganteum T, 1.5 ppm and 30 oz/acre, 2 × (20 day interval) CTS 119–194 m2 (4) − 38.5 (− 80.7, + 95.7)
 Teng et al. (2005) Taiwan An. sinensis Lagenidium giganteum A, 1.5 ppm and 30 oz/acre, 2 × (20 day interval) CTS 119–194 m2 (4)  + 1.3 (− 69.0, + 231.3)
 Balaraman et al. 1983) India An. subpictus Bti serotype H-14 (VCRC B-17), with dose 27 × 105 spores/mL, 3 × CTS 1000 m2 (5) − 75.8 (− 87.0, − 55.0)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Abbott 6108b 300 T.U./mg), at 6.0 kg/ha, 3 × CTS 30 m2 (3) − 42.3 (− 58.1, − 20.4)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Abbott 6108b 300 T.U./mg), at 3.0 kg/ha, 3 × CTS 30 m2 (3) − 60.8 (− 66.2, − 54.5)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Abbott 6108b 300 T.U./mg), at 1.5 kg/ha, 3 × CTS 30 m2 (3) − 42.3 (− 58.5, − 19.7)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Biochem-Bactimos 666 1800 T.U./mg), at 1.0 kg/ha, 3 × CTS 30 m2 (3) − 30.0 (− 48.8, − 4.3)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Biochem-Bactimos 666 1800 T.U./mg), at 0.5 kg/ha, 3 × CTS 30 m2 (3) − 29.1 (− 41.4, − 14.2)
 McLaughlin et al. (1982) USA An. crucians Bti, H-14 (Biochem-Bactimos 666 1800 T.U./mg), at 0.25 kg/ha, 3 × CTS 30 m2 (3) − 23.2 (− 38.4, − 4.4)
RE model for all studies − 60.0 (− 71.8, − 43.1)
 Bolay & Trpis (1989) Liberia An. gambiae s.l Bti, Teknar HP-D, at 0.1 g/m2 CITS 150 m2 (3) − 75.8 (− 96.0, + 46.3)
 Yu et al. (1993) S. Korea An. sinensis Bti, H-14 (Bactis-P), at 0.1 kg/ha CITS 1000 m2 (6) − 67.6 (− 97.0, + 251.1)
RE model for all studies − 76.3 (− 95.4, + 21.9)
Larviciding and biological control
Bacterial larvicide and fish
 Kramer et al. (1988) USA An. freeborni Bti, Vectobac (200 ITU/mg), at 6 kg/ha + Gambusia affinis at 1.1 kg/ha CTS 1000 m2 (3) − 31.0 (− 68.3, + 50.3)
 Kramer et al. (1988) USA An. freeborni Bti, Vectobac (200 ITU/mg), at 6 kg/ha + G. affinis at 3.4 kg/ha CTS 1000 m2 (3) − 82.8 (− 91.9, − 63.4)
RE model for all studies − 65.7 (− 91.2, + 34.2)
 Bolay & Trpis (1989) Liberia An. gambiae s.l Bti, Teknar HP-D, at 0.1 g/m2 + Tilpania nilotica (300) CITS 150 m2 (3) − 88.1 (− 96.1, − 63.9)
 Yu & Lee (1989) S. Korea An. sinensis Bti, H-14, at 1 kg/ha + Aplocheilus latipes at 2/m2 CITS 150 m2 (2) − 67.0 (− 79.8, − 46.2)
RE model for all studies − 88.0 (− 95.0, − 71.3)
Biological control
Fish
 Kramer et al. (1988) USA An. freeborni G. affinis at 1.1 kg/ha CTS 1000 m2 (3) − 77.7 (− 88.2, − 56.1)
 Kramer et al. (1988) USA An. freeborni G. affinis at 3.4 kg/ha CTS 1000 m2 (3) − 88.6 (− 94.2, − 77.9)
 Victor et al. (1994) India An. subpictus 3 indigenous carps (Catla catla, labeo rohita, cirrhinus mrigala) + 3 exotic carps (Cyprinus carpio, Hypopthalmithys molitri, Ctenopharyngodon idella) stocked at rate of 10,000/ha CTS 400 m2 (3) − 51.6 (− 76.2, − 1.6)
 Yu et al. (1981) S. Korea An. sinensis Aphycypris chinensis (presence) CTS 2000 m2 (2) − 92.2 (− 97.3, − 77.2)
RE model for all studies − 81.5 (− 91.4, − 60.2)
 Bolay & Trpis (1989) Liberia An. gambiae s.l Tilapia nilotica (n = 300) CITS 150 m2 (3) − 87.8 (− 96.0, − 62.4)
 Kim et al. (2002) S. Korea An. sinensis Tilapia mossambicus at 2 fish/10 m2 CITS 300–600 m2 (2–4) − 41.8 (− 57.1, − 20.9)
 Kim et al. (2002) S. Korea An. sinensis A. chinensis at 2 fish/10m2 CITS 300–600 m2 (2–4) − 62.4 (− 76.0, − 41.2)
 Kim et al. (2002) S. Korea An. sinensis T. mossambicus at 2 fish/10m2 + A. chinensis at 1/m2 CITS 300–600 m2 (2–4) − 55.1 (− 72.6, − 26.3)
 Yu & Lee (1989) S. Korea An. sinensis A. latipes at 2 fish/m2 + T. mossambicus at 2/m2 CITS 150 m2 (2) − 73.4 (− 80.5, − 63.6)
RE model for all studies − 87.1 (− 93.9, − 72.7)
Copepod
 Marten et al. (2000) USA An. Quadrimaculatus Mesocyclops ruttneri (n = 500) CTS 100 m2 (2) − 40.5 (− 82.8, + 105.6)
Azolla
 Rajendran & Reuben (1991) India An. subpictus Azolla microphylla introduced at rate 100 g/m2 on 5th DAT3 CTS 40 m2 (2) − 48.7 (− 96.8, + 720.4)
 Rajendran & Reuben (1991) India An. subpictus Azolla microphylla introduced at rate 200 g/m2 on 5th DAT CTS 40 m2 (2)  + 45.6 (− 89.0, + 1826.3)
RE model for all studies -10.3 (-86.4, + 493.3)
Neem
 Rao et al. (1995) India An. subpictus Neem (Nimin) at 0.063 kg ai/ha CTS 400 m2 (3) − 29.4 (− 84.3, + 217.8)
 Rao et al. (1995) India An. subpictus Neem (Nimin)-coated urea at 0.063 kg ai/ha + 62.5 kg urea/ha CTS 400 m2 (3) − 34.0 (− 74.4, + 70.4)
 Rao et al. (1995) India An. subpictus Neem-coated urea (Neemrich-1 80EC4) at 0.09 kg ai/ha CTS 400 m2 (3) − 25.1 (− 75.4, + 127.7)
 Rao et al. (1995) India An. subpictus As above + 62.5 kg urea/ha CTS 400 m2 (3) − 33.2 (− 83.5, + 171.2)
 Rao et al. (1995) India An. subpictus Neem-coated urea (Neemrich-1 80EC) at 0.12 kg ai/ha CTS 400 m2 (3) − 27.0 (− 81.5, + 187.4)
 Rao et al. (1995) India An. subpictus As above + 62.5 kg urea/ha CTS 400 m2 (3) − 32.6 (− 76.6, + 93.9)
RE model for all studies − 30.7 (− 57.2, + 12.3)
Azolla and neem
 Rajendran & Reuben (1991) India An. subpictus Azolla microphylla at 100 g/m2 on 5th DAT + neem cake powder 50 g/m2 on day of transplantation (TP) CTS 40 m2 (2) − 53.9 (− 96.5, + 528.2)
Neem and water management technique
 Rao et al. (1995) India An. subpictus Neem (Nimin)-coated urea at 0.063 kg ai/ha + 62.5 kg urea/ha + water allowed to stand 2.5–3.5 cm in the week following TP + from the second week, plots were dried for 2–3 days before re-irrigation CTS 400 m2 (3) − 27.5 (− 90.1, + 430.6)
 Rao et al. (1995) India An. subpictus Neem-coated urea (Neemrich-1 80EC) at 0.09 kg ai + 62.5 kg urea/ha + water allowed to stand 2.5–3.5 cm in the week following TP + from the second week, plots were dried for 2–3 days before re-irrigation CTS 400 m2 (3) − 43.7 (− 93.3, + 370.7)
RE model for all studies − 35.6 (− 84.9, + 175.2)

Significant values are in bold.

*The number of plots per treatment group.

1CTS: Controlled time series.

2CITS: Controlled interrupted time series.

3DAT: Days after transplanting.

4EC: Emulsifiable concentrate.