Fig. 2. SAA improve movement and increase H₂S levels in dys-1(eg33) animals.

a L-methionine treatment significantly improved thrashing rates of dys-1(eg33) animals in a dose-dependent manner (1–10 mM). b L-homocysteine improved movement in dys-1(eg33) (10 µM–1 mM). c L-cysteine significantly improvement thrashing in dys-1(eg33) (10 µM–5 mM). d dys-1(eg33) movement was improved with L-glutathione treatment (10 µM–5 mM). e L-taurine treatment also improved thrash rate in dys-1(eg33) (10 µM–5 mM). f Summary figure of the selected optimum doses for each SAA, there are no significant differences between amino acids. Phenylalanine improves movement in the dys-1(eg33) model but not to the same levels as the SAA. There is no improvement with lysine treatment. For all strains and treatment, 10 animals were assayed and averages taken from 5 separate counts and repeated across three biological repeats for a total of 150 data points per violin plot. Results were analysed with a two-way ANOVA. All significance points were compared to dys-1(eg33) ***P < 0.0001, **P < 0.005, *P < 0.05, ns P > 0.05. g Fluorescence intensity of the AzMC signal, normalised to protein content. All SAA increased H₂S levels. Data for each treatment are from 270 animals across three biological repeats. Results were analysed with a one-way ANOVA. All significance points are compared to dys-1(eg33). ***P < 0.0001.