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. 2022 Nov 3;13:1057746. doi: 10.3389/fimmu.2022.1057746

Figure 7.

Figure 7

Specific depletion of NLRP3 in macrophages alleviates the severity of cystitis in diabetic mice. We generated mice with macrophage-depletion of NLRP3, Lys2-Cre; NLRP3(fx/fx) and control NLRP3(fx/fx). (A) Flow cytometry showing isolation of macrophages from the bladders of these mice. (B) ELISA for NLRP3 in macrophages nor non-macrophages from the mouse bladder. (C) STZ was used at D0 to develop diabetes in mice. After 6 days when diabetes was confirmed, experimental cystitis was induced by 4 i.p. injection of CYP on D6, 8, 10 and 12. Von Frey test was done at D13, and mice were sacrificed at D16 for end assessments. Group 1: NLRP3(fx/fx) mice received i.p. saline (control for STZ) at D0 and i.p. CYP at D6, 8, 10 and 12 (saline+CYP/N); Group 2: Lys2-Cre; NLRP3(fx/fx) mice received i.p. saline (control for STZ) at D0 and i.p. CYP at D6, 8, 10 and 12 (saline+CYP/LN); Group 3: NLRP3(fx/fx) mice received i.p. STZ at D0 and i.p. CYP at D6, 8, 10 and 12 (STZ+CYP/N); Group 4: Lys2-Cre; NLRP3(fx/fx) mice received i.p. STZ at D0 and i.p. CYP at D6, 8, 10 and 12 (STZ+CYP/LN). (C) Nociceptive score of the mice at Von Frey test at D13. (D) Changes in body weight. (E) Bladder weight at D16. (F) Edema score of the bladder at D16. (G) Vesical vascular permeability in bladder at D16. *p<0.05. ns: no significance.