TABLE 2.

General statistics of the 16S rRNA gene sequencing for all samples.

Sample	Raw reads	Clean reads	Bases (nt)	AvgLen (nt)	Q20	GC%	Effective%
SLCql1	64074	58032	24666390	425	83.61	53.05	90.57
SLCql2	63759	59655	25335392	424	84.39	53.63	93.56
SLCql3	87039	80056	34112373	426	84.07	53.66	91.98
SLCql4	78309	73403	30519852	415	82.97	54.48	93.74
SLCql5	50799	45892	19194215	418	82.77	53.97	90.34
SLCql6	80288	72950	30800498	422	82.96	53.90	90.86
Total	424268	389988	-	-	-	-	-
Mean	70711.33	64998	27438120	421.67	83.46	53.78	91.84
RLCql1	87479	80144	34089687	425	84.97	52.29	91.62
RLCql2	83317	75745	31695677	418	83.19	51.70	90.91
RLCql3	84472	80071	33540979	418	82.35	52.29	94.79
RLCql4	85812	80330	34201426	425	84.64	52.00	93.61
RLCql5	74702	67384	27855108	413	85.29	54.12	90.20
RLCql6	87895	80208	33918008	422	84.68	53.45	91.25
Total	503677	463882	-	-	-	-	-
Mean	83946.17	77313.67	32550148	420.17	84.19	52.64	92.06

Raw reads, sequences after filtering out low-quality bases; Clean reads, sequences that are finally used for subsequent analysis after filtering out chimeras; Bases, the number of bases in the final Clean reads; AvgLen, the average length of Clean reads; Q20, the percentage of bases with a base mass value > 20 (sequencing error rate < 1%) in Clean reads; GC (%), the content of GC bases in Clean reads; Effective (%), the percentage of the number of Clean reads out of the number of Raw reads.