Figure 1.

Comparison of mouse and human utrophin promoter A sequences. The regions of interest in the 1.3 kb HindIII human promoter A region (EMBL accession no. X95523; top) and sequence conservation with the mouse region (EMBL accession no. X95524; bottom) are shown. The N-box and conserved E-box motifs are boxed, with restriction sites used in footprinting and luciferase constructs underlined. Regions that bind Sp1 or Ap2 are indicated in black, with a plus symbol indicating the Sp1 region unable to be mutated in this study. Grey regions refer to sites that did not bind either factor in EMSA studies. The multiple transcription start sites of the human sequence (15) are marked by asterisks.