Table 1.
List of Reagents and Platforms.
| Reagent/Item Description | Reference number |
|---|---|
| HL-60 cell line | ATCC, cat. no. CCL-240 |
| IMDM | Gibco, cat. no. 12440061 |
| FBS | ATCC, cat. no. 30–2020 |
| Penicillin + Streptomycin | Gibco, cat. no. 15140122 |
| Cell Imaging Cytometer | Nexcelom, CeligoS, cat. no. 200-BFFL-S |
| Agilent Bioanalyzer 2100 | Agilent, model G2939A |
| QuBit 2.0 | Invitrogen, cat. no. Q32866 |
| Qiagen AllPrep DNA/RNA/Protein Minikit | Qiagen, cat. no. 80004 |
| Ultracentrifuge tube | cat. no. 149569 C |
| On-Chip Sort Flow Cytometer-2D Chip-Z1001 | cat. no. 1002004 |
| MitoSOX™ Red Mitochondrial Superoxide Indicator | Invitrogen, cat. no. M36008, lot 2015529 |
| Eosin Y, 0.25% (w/v) in 57% Alcohol | Ricca Chemical, cat. no. 284516 |
| Hematoxylin Stain Solution, Gill 1 formulation, regular strength | Ricca Chemical, cat. no. 353516 |
| Giemsa Stain Solution | LabChem, Inc., cat. no. LC148407 |
| Confocal Microscope | Leica TCS SP5 II |
| Cytofix/Cytoperm Fixation and Permeabilization solution | BD Biosciences, cat. no. 55472251-2090KZ |
| DAPI | Invitrogen, cat. no. D1306 |
| Cytoseal-60 mounting media | Thermo Scientific, cat. no. 83104 |
| Tomographic Microscope | Nanolive 3D Cell Explorer |
| Scanning Electron Microscope | Leica UCT Ultramicrotome |
| Grid Viewing Microscope | Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera (FEI) |
| Formvar-carbon-coated copper grids | 100 mesh, Electron Microscopy Sciences, Hatfield, PA) uranyl acetate (Ladd Research Industries, Williston, VT |
| Grid Examination | EOL JEM-1400Plus transmission electron microscope operating at 80 kV |
| Grid Recording Camera | Gatan OneView 4 K |
| NexteraXT library prep kit | Illumina, cat. no. FC-131-1096 |
| Sequencing Platform | Illumina, NovaSeq 6000 |
| Flow Cell | S2 2X150bp |
| DNA Mapping Software | CLC Workbench v11, v12 |
| Neutrophil Elastase ELISA | Abcam, cat. no. ab204730 |