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. 2022 Nov 16;117(1):61. doi: 10.1007/s00395-022-00970-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — PLC have no major impact on infarct size and cardiac function post-myocardial infarction. A Fractions of PMC, and PNC before and 24 h after myocardial I/R injury in WTWT and KOWT chimeras. WTWT chimeras, *p < 0.05 denote statistically significant differences between time points within the same group and ^§p < 0.05 between groups at the same time point. Results are presented as mean ± SEM, n = 8 per group, two-way ANOVA). B Blood counts for monocytes (Mono) and neutrophils (Neutro) 24 h after myocardial I/R injury. Results are presented as mean ± SEM, n = 8 per group. C Representative dot plots showing gating for leukocyte populations in enzymatically digested infarcted myocardium (left) and quantification of Ly6C^high monocytes, neutrophils, PMC and PNC, and macrophages. Results are presented as mean ± SEM, *p < 0.05 denote statistically significant differences between groups, n = 9 for WTWT and n = 8 for KOWT, t test). D High-sensitive Troponin T plasma levels in WTWT and KOWT chimeras 24 h after myocardial I/R injury. Results are presented as mean ± SEM, n = 8 per group, t test. E Representative images of left ventricle (LV) sections stained with 2,3,5-triphenyltetrazolium chloride (TTC) and quantification of the area at risk (AAR) and the infarcted area within (infarct). Results are presented as mean ± SEM, n = 10 for WTWT, n = 9 for KOWT. F Representative image of anti-Ly6G stained infarct areas and quantification of mean Ly6G + neutrophil numbers per LV section (ten sections analyzed per mouse). Results are presented as mean ± SEM, n = 8 per group, t test. G Echocardiographic analysis (representative pictures of B- and M-Mode for left-ventricular end-systolic and end-diastolic dimensions depicted) before and 21 days after myocardial I/R injury in WTWT and KOWT chimeras. Results are presented as mean ± SEM, n = 5 per group at d0, and n = 8 per group at d21, Two-way ANOVA). H Heat map depicting qRT-PCR-based expression levels of key genes relative to mean values of the respective genes in WTWT chimeras (blue = lowest, white = mean; red = highest gene expression) n = 8 per group; p values calculated with multiple t-testing and Bonferroni correction, and p < 0.05 denoting statically significant differences between groups