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. 2022 Sep 8;37(8):2677–2685. doi: 10.1007/s11011-022-01021-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

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Fig.3 — Western blot analysis to evaluate the effect of cell transplantation and melatonin treatment on the expression levels of an apoptotic marker Caspase-3 in 6-OHDA-injected rats. A Western blot for cleaved caspase-3 are shown. Equal amounts of total proteins were separated by SDS-PAGE and blots were probed with anti-caspase-3 and anti β-actin antibody. The results showed high expression of cleaved caspase-3 in 6-OHDA-injected rats. B The densities of cleaved caspase-3 bands were measured and the ratios to βactin bands were calculated. Number sign indicates the difference between the 6-OHDA-injected, treatment and control groups (*, P < 0.05; **, P < 0.01; ***, P < 0.001)