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. 2022 Nov 3;9:1000578. doi: 10.3389/fcvm.2022.1000578

TABLE 1.

Introduction to the composition, localization and related functions of NADPH oxidase.

Essential regulatory subunits Requirement for p22phox Cell/tissue distribution Cardiomyocyte expression Regulating factors Model building References
NOX1 NOXO1
NOXA1
RAC
Yes Smooth muscle cells
Fibroblasts
Endothelial cells
No AngII NF-κB
ET-1 IFN-γ
VEGF PDGF
ATF-1 GPER
ApoE–/–, SHRs
TgSMCnox1
HFHS/STZ, NoxO1–/–
Gper–/–
(6, 812, 17, 20, 22, 26, 27, 29, 33, 3639)
NOX2 Gp91Phox
p47Phox
p67Phox
p40Phox
RAC
Yes Cardiomyocytes, endothelial cells
Vascular-smooth muscle cells
Fibroblasts
Yes AngII ET-1
TGF-β IFN-γ
PDGF VEGF
Nox2–/–, ApoE–/–
LysMCre/WT
gp91phox–/– TLR5–/–
TRPC3/C6 ectopic-
expression
(6, 1012, 17, 20, 31, 34, 41, 42, 44, 45, 47, 51, 52, 59, 104)
NOX3 NOXO Yes Fetal tissue inner ear No (11, 12)
NOX4 Calcium ions Yes Cardiomyocytes, endothelial cells
Fibroblasts
Vascular-smooth muscle cells
Yes HIF-1 TGF-β
TNF-α IFN-γ
VEGF
NOX4–/–, APOE–/–
Tg-Nox4 HCAEC
Cardio-Nox4–/–
Endo-Nox4–/–
ApoE–/–/ p47phox–/–
SHRs
(6, 7, 9, 22, 31, 35, 62, 63, 6770, 75)
NOX5 Calcium ions No Endothelial cells
Vascular-smooth muscle cells
Extravascular fibroblasts
No AngII TNF-α
TGF-β PDGF
IFN-γ
NOX5±CRE±TeloHAEC cell line
NOX5-siRNA
(6, 17, 20, 22, 31, 7779, 81, 82, 84)

AngII, Angiotensin II; NF-K b, nuclear factor kappa-B; ET-1,endothelin-1; IFN-γ, interferon-γ; VEGF, vascular endothelial growth factor; PDGF, platelet derived growth factor; ATF-1, activating transcription Factor 1; GPER, G-protein-coupled estrogen receptor; HIF-1, hypoxia-inducible factor-1; TNF, tumor necrosis factor; TGF-β, transforming growth factor-β; ApoE–/–, ApoE knockout mice; SHRs, spontaneously hypertensive rats; TgSMCnox1, mice overexpressing Nox1 in SMCs; HFHS/STZ, high-fat and high-sugar diet/streptozotocin mouse model; NoxO1–/–, Noxo1-knockout mice; Gper–/–, G protein-coupled estrogen receptor gene deficient mice; Nox2–/–, Nox2 knockout mice; LysMCre/WT, Model mice using the promoter of LysM gene to drive Cre recombinase; gp91phox–/–, gp91phox knockout mice; TRPC3/C6, Canonical transient receptor potential; CRE, Cyclization Recombination Enzyme; Cardio-Nox4–/–, Cardiomyocyte NOX4 knockout mice; Endo-Nox4–/–, endothelial cell NOX4 knockout mice; Tg-Nox4 HCAEC, NOX4 overexpression in mouse coronary endothelial cells; TeloHAEC cell line, is a clonal cell line immortalized by stably expressing human telomerase catalytic subunit hTERT; NOX5-siRNA, NOX5-silenced.