FIGURE 2.

Effect of STAT3 on cellular proliferation and glycolytic rate. (A) Representative western blot confirming STAT3 deletion in canine OS cell lines. (B) Proliferation over 72 h was determined in STAT3 knockout lines using the SRB assay. Results are presented as the mean + SD across three biologic replicates. p < .05 was considered significant. (C) Basal and compensatory glycolysis were determined in STAT3 knockout cell lines using the Seahorse XFe Glycolytic Rate Assay. Results of one representative biologic replicate are shown as mean ± SD. p < .01 was considered significant. M(#) = monoclonal cell line. ⁺ p < .01; ^# p ≤ .001