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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Nat Biomed Eng. 2022 Aug 8;6(11):1284–1297. doi: 10.1038/s41551-022-00915-0

Extended Data Fig. 5. T lineage commitment of TRAC-CAR-TiPS.

Extended Data Fig. 5.

a, T lineage commitment marker expression (CD7/CD5, CD4/CD8α, CD8α/CD8β) of WT-TiPS (left), TRAC-1928z-TiPS (middle) and TRAC-1XX-TiPS on OP9-DLL4 at the indicated timepoints. CD7/CD5 is gated on live CD45+ cells, others are gated on live CD45+CD7+ cells. b, Flow cytometric analysis of T cell phenotype markers of D35 DP TRAC-1XX-iT cells. Gated on live CD45+CD7+CD4+CD8αβ+ cells. c, Intracellular and cell-surface expression of CD3 and TCRαβ on D35 TRAC-1XX-iT cells.