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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Nat Biomed Eng. 2022 Aug 8;6(11):1284–1297. doi: 10.1038/s41551-022-00915-0

Extended Data Fig. 1. T lymphoid commitment of hES, FiPS and TiPS on OP9-mDLL1.

Extended Data Fig. 1.

a, Flow cytometric analysis of pluripotency marker expression on H1, FiPS and WT-TiPS b, Flow cytometric analysis of T lymphoid markers of H1, FiPS and WT-TiPS during differentiation on OP9-mDLL1 at indicated timepoints. Plots depicting CD7/CD5 are gated on live CD45+ cells, plots depicting CD3/TCRαβ, CD4/CD8α and CD8α/CD8β are gated on live CD45+CD7+ cells. CD3/TCRαβ and CD4/CD8α at D40 are as presented in Fig. 1b.