KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC-Cy7-anti-human CD69 | BD Pharmingen | 557756 |
| FITC Mouse Anti-Human CD4 Clone M-T477 | BD Biosciences | 556615 |
| Pacific Blue™ Mouse Anti-Human CD8 | BD Biosciences | 558207 |
| Dynabeads™ Human T-Activator CD3/CD28 | Thermo Fisher Scientific | 11132D |
| Alexa Fluor 647 anti-V5 Tag | Invitrogen | 451098 |
| Alexa Fluor 488- anti-myc Tag | R&D Systems | IC3696G |
| Cell Culture Reagents | ||
| RPMI-1640 | Lonza | 12-702Q |
| Ultraculture | Lonza | 12-725F |
| DMEM | Corning | 10-013 |
| X-VIVO 15 | Lonza | 04-418Q |
| Human AB serum | Valley Medical | HP1022 |
| FBS | Thermo Fisher Scientific | 10437-028 |
| 2-Mercaptoethanol | Thermo Fisher Scientific | 31350010 |
| L-glutamine | Corning | 25-005-CL |
| Penicillin/Streptomycin | Caisson | PSL01 |
| Sodium Butyrate | Alfa Aesar | A11079 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Grazoprovir | MedChemExpress | HY15298 |
| Grazoprovir potasium salt | MedChemExpress | HY-15298A |
| Danoprevir | ApexBio | A4024 |
| Glecaprevir | MedChemExpress | HY-17634 |
| Simeprevir | MedChemExpress | HY-10241 |
| Boceprevir | MedChemExpress | HY-10237 |
| TMP | Sigma-Aldrich | 92131 |
| Lenalidomide | MedChemExpress | HY-A0003 |
| Pomalidomide | MedChemExpress | HY-10984 |
| XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate | Perkin Elmer | 122799 |
| N-Acetyl-L-cysteine | Sigma-Aldrich | A9165 |
| Lenti-X™ Concentrator | Clontech | 631232 |
| RetroNectin® Recombinant Human Fibronectin Fragment | Clontech | T100B |
| Phosphate-Buffered Saline (10X) pH 7.4 | Thermo Fisher Scientific | AM9625 |
| PEG8000 | Thermo Fisher Scientific | BP233-1 |
| Sodium Chloride | Thermo Fisher Scientific | AAJ2161836 |
| DMSO | CHEM-IMPEX INT’L INC | 00635 |
| PEG400 | MedChemExpress | HY-Y0873A |
| Corn oil | MedChemExpress | HY-Y1888 |
| Critical Commercial Assays | ||
| RosetteSep™ Human CD4+ T Cell Enrichment Cocktail | STEMCELL Technologies | 15062 |
| RosetteSep™ Human CD8+ T Cell Enrichment Cocktail |
STEMCELL Technologies | 15063 |
| EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit | STEMCELL Technologies | 18063 |
| Human IL-2 ELISA Set | BD Biosciences | 555190 |
| Human IFN-γ ELISA Set | BD Biosciences | 555142 |
| XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate | PerkinElmer | 122799 |
| CellTrace™ Violet Cell Proliferation Kit, for flow cytometry | Thermo Fisher Scientific | C34557 |
| RosetteSep™ Human CD4+ T Cell Enrichment Cocktail | STEMCELL Technologies | 15062 |
| Experimental Models: Cell Lines | ||
| HEK293FT | N/A | N/A |
| NFAT-Jurkat | Art Weiss (UCSF) | N/A |
| NALM6 (Luc+ BFP+) | This paper | N/A |
| NALM6 (HER2+ Axl+ Mesothilin+ Luc+) | This paper | N/A |
| NALM6 (HER2+ mCherry+) | This paper | N/A |
| NALM6 (Axl+ GFP+) | This paper | N/A |
| MSTO-211H (Luc+ BFP+) | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (female 6–8 weeks) | Jackson Laboratory | 005557 |
| Scid-beige(female 6–8 weeks) | Taconic Biosciences | CBSCBG-F |
| Recombinant DNA | ||
| pHR-SFFV vector | Addgene | 79121 |
| PB-pCAG-MCS-EF1a-zeocine-BFP vector | This paper | N/A |
| Software and Algorithms | ||
| Graph Pad Prism 7 | Graph Pad | N/A |
| FlowJo V10 | TreeStar | N/A |
| Living Image | Perkin Elmer | N/A |