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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Oncogene. 2022 Oct 11;41(46):4983–4993. doi: 10.1038/s41388-022-02492-7

Figure 2. Stat3 co-immunoprecipitates with Ezh2 in Ezh2Y641F mutant cells and is hypermethylated.

Figure 2.

(a) Top: Representative image of immunoblotting analysis for Stat3 and actin in the cytoplasm, and Stat3 and phospho-Stat3 in the nucleus of mouse melanoma cell lines 480 and 855 (Δ = Y641F mutant). The bar graph represents quantification of the Stat3 nuclear/cytoplasmic ratio and is plotted as average ± SD (N = 4/group, p = 0.051).

(b) Immunoprecipitation for Stat3 and Ezh2, immunoblotted for Stat3, Ezh2, and tri-methylated lysine.