Figure 4. Changes in global chromatin binding by Ezh2 and Stat3 in Ezh2^Y641F melanoma cells and its effect on gene expression.

(a) ChIP-seq peaks for Ezh2 (left circle) and Stat3 (right circle) in Ezh2^Y641F melanoma cells reveal new binding sites for Ezh2 and Stat3, and sites co-occupied by both proteins.

(b) Transcription factor binding motifs enriched in the co-occupied peaks of Ezh2 and Stat3 in Ezh2^Y641F melanoma cells from panel (a).

(c) ChIP-seq peaks of Ezh2, Stat3 and H3K27me3 in Ezh2^WT and Ezh2^Y641F melanoma cells, proximal to the Ezh2 gene locus on chromosome 7.

(d) Expression of Ezh2 in Ezh2^WT vs Ezh2^Y641F melanoma cells after Stat3 knockdown (***p<0.001, *p<0.05).

(e) Sequential ChIP-reChIP with Ezh2 and Stat3 at the co-occupied peaks in Ezh2^Y641F melanoma cells (*p<0.05, **p<0.01).