Table 3.
Stability of RNA helices in E. coli metabolite mixtures.
| Sequencea | AU content (%) | Conditionb | ΔG°37 (kcal/mol)c | ΔΔG°37 (kcal/mol)c |
|---|---|---|---|---|
| 1: 5’-CGGAUGGC-3’ 3’-GCCUACCG-5’ |
25% | Background | −15.60 (0.23) | -- |
| Eco80 | −14.99 (0.22) | +0.61 (0.32) | ||
| NTPCM | −15.28 (0.23) | +0.32 (0.33) | ||
| WMCM | −15.23 (0.23) | +0.37 (0.33) | ||
| 2: 5’-CGCAUCCU-3’ 3’-GCGUAGGA-5’ |
38% | Background | −13.84 (0.21) | -- |
| Eco80 | −12.70 (0.19) | +1.17 (0.28) | ||
| NTPCM | −13.42 (0.20) | +0.42 (0.29) | ||
| WMCM | −14.22 (0.21) | −0.39 (0.30) | ||
| 3: 5’-CGUAUGUA-3’ 3’-GCAUACAU-5’ |
63% | Background | −10.85 (0.16) | -- |
| Eco80 | −10.41 (0.16) | +0.44 (0.23) | ||
| NTPCM | −10.30 (0.15) | +0.55 (0.22) | ||
| WMCM | −10.85 (0.16) | 0.00 (0.23) | ||
| 4: 5’-CCAUAUCA-3’ 3’-GGUAUAGU-5’ |
63% | Background | −12.03 (0.18) | -- |
| Eco80 | −11.38 (0.17) | +0.64 (0.25) | ||
| NTPCM | −11.50 (0.17) | +0.52 (0.25) | ||
| WMCM | −11.53 (0.17) | +0.50 (0.25) | ||
| 5: 5’-CCAUAUUA-3’ 3’-GGUAUAAU-5’ |
75% | Background | −10.76 (0.16) | -- |
| Eco80 | −10.15 (0.15) | +0.61 (0.22) | ||
| NTPCM | −10.16 (0.15) | +0.60 (0.22) | ||
| WMCM | −9.96 (0.15) | +0.80 (0.22) |
a
The first sequence in each set was 5′-FAM labeled while the second sequence was 3′-BHQ1 labeled.
b
All solutions contain 2 mM Free Mg2+, 240 Na+, 140 mM K+.
c
Extra significant digits were included to avoid propagating rounding errors