KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-GLS1 (KGA/GAC) polyclonal antibody | Proteintech | Cat# 12855-1-AP; RRID:AB_2110381 |
| anti-pyruvate carboxylase polyclonal antibody | Proteintech | Cat# 16588-1-AP; RRID:AB_1851513 |
| anti-β-actin monoclonal antibody | Sigma | Cat# A1978; RRID:AB_476692 |
| IRDye 680RD donkey anti-mouse | LI-COR | Cat# 926-68072; RRID:AB_10953628 |
| IRDye 800CW donkey anti-rabbit | LI-COR | Cat# 926-32213; RRID:AB_621848 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| [18F]fluoroglutamine | Memorial Sloan Kettering Cancer Center’s Radiochemistry and Molecular Imaging Probe Core Facility | N/A |
| CB-839 | Selleckchem | Cat# S7655 |
| BPTES | Selleckchem | Cat# S7753 |
| Menadione | Selleckchem | Cat# S1949 |
| Buthionine sulfoximine | Sigma | Cat# 5.08228 |
| DMEM/F-12 | USBiological | Cat# D9807-06 |
| Dialyzed FBS | Gibco | Cat# 26400044 |
| D-glucose | Corning | Cat# 25-037-Cl |
| L-glutamine | Gibco | Cat# 25030081 |
| Sulforhodamine B | Sigma | Cat# S1402 |
| H2O2 | Sigma | Cat# H1009 |
| N-acetyl-cysteine | Sigma | Cat# A9165 |
| Dimethyl-α-ketoglutarate | Sigma | Cat# 349631 |
| Sodium pyruvate | Sigma | Cat# P5280 |
| [13C6]glucose | Cambridge Isotope Laboratories | Cat# CLM-1396-PK |
| [13C515N2]glutamine | Cambridge Isotope Laboratories | Cat# CNLM-1275-H-PK |
| Methanol (MS grade) | Thermo Fisher Scientific | Cat# A456 |
| Water (MS grade) | Sigma | Cat# 900682 |
| Acetonitrile (MS grade) | Sigma | Cat# 900667 |
| Methoxyamine hydrochloride | Sigma | Cat# 226904 |
| Pyridine anhydrous 99.8% | Sigma | Cat# 270970 |
| MTBSTFA (with 1% t-BDMCS) | Sigma | Cat# M-108 |
| cOmplete protease inhibitor cocktail | Roche | Cat# 11697498001 |
| PhosStop phosphatase inhibitor cocktail | Roche | Cat# 4906845001 |
| NuPAGE LDS sample buffer | Thermo Fisher | Cat# NP0007 |
| NuPAGE sample reducing agent | Thermo Fisher | Cat# NP0004 |
| MOPS SDS running buffer | Thermo Fisher | Cat# NP0001 |
| Odyssey nitrocellulose membrane | LI-COR | Cat# 926-31092 |
| Intercept blocking buffer PBS | LI-COR | Cat# 927-70003 |
| Hygromycin B | Goldbio | Cat# H-270-1 |
| D-luciferin firefly | Biosynth Carbosynth | Cat# L-8220 |
| hEGF | Sigma | Cat# E9644 |
| Matrigel basement membrane matrix | Corning | Cat# 354234 |
| [13C6]citric acid | Cambridge Isotope Laboratories | Cat# CLM-9021-PK |
| [13C4]α-ketoglutaric acid sodium salt | Cambridge Isotope Laboratories | Cat# CLM-4442 |
| [13C4]succinic acid | Cambridge Isotope Laboratories | Cat# CLM-1571 |
| [13C4]fumaric acid | Cambridge Isotope Laboratories | Cat# CLM-1529 |
| [13C4]L-malic acid | Cambridge Isotope Laboratories | Cat# CLM-8065 |
| Stable isotope labeled canonical amino acid mix | Cambridge Isotope Laboratories | Cat# MSK-CAA-1 |
| RIPA lysis buffer cocktail | Santa Cruz | Cat# sc-24948 |
| 5-sulfosalicylic acid | Sigma | Cat# S7408 |
| Dihydroethidium stain | Thermo Fisher | Cat# D11347 |
| Hoechst 33342 | Thermo Fisher Scientific | Cat# 62249 |
| Propidium iodide | Sigma | Cat# P4170 |
| Annexin V, pacific blue conjugate | Thermo Fisher Scientific | Cat# A35122 |
| Triton-X-100 | Sigma | Cat# 9002-93-1 |
| RNAse A, DNase and protease-free | Thermo Fisher Scientific | Cat# EN0531 |
| Annexin binding buffer | Thermo Fisher Scientific | Cat# V13246 |
| OptiMEM | Gibco | Cat# 31985070 |
| FuGENE 6 Transfection Reagent | Promega | Cat# E2691 |
| Polybrene | Santa Cruz | Cat# SC-1342200 |
| Sodium butyrate | Sigma | Cat# B5887 |
| Puromycin | Sigma | Cat# P8833 |
| Critical commercial assays | ||
| DC protein assay kit | Bio-Rad | Cat# 5000111 |
| Pierce BCA assay kit | Thermo Fisher Scientific | Cat# 23225 and Cat# 23227 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| Nf1−/−; DNp53 astrocytes | Rubin Laboratory | N/A |
| Nf1−/−; DNp53; PC KD astrocytes | This manuscript | N/A |
| HEK 293T cells | Kroll Laboratory | N/A |
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| pLKO.1-puromycin-LacZ shRNA | McDonnell Genome Institute at Washington University in St. Louis | NA |
| pLKO.1-puromycin-PC_1 shRNA | Sigma | Cat# TRCN0000112425 |
| pLKO.1-puromycin-PC_2 shRNA | Sigma | Cat# TRCN0000112426 |
| pLKO.1-puromycin-PC_3 shRNA | Sigma | Cat# TRCN0000112427 |
| pLKO.1-puromycin-PC_4 shRNA | Sigma | Cat# TRCN0000112428 |
| Software and algorithms | ||
| R v4.1.1 | R Core Team, 2021 | https://www.r-project.org/ |
| Limma v3.48.3 | Bioconductor |
https://bioconductor.org/packages/release/bioc/html/limma.html
DOI:10.18129/B9.bioc.limma |
| PET VCAR software | GE Healthcare | https://www.gehealthcare.com/products/advanced-visualization/all-applications/pet-vcar |
| GraphPad Prism v9 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| Skyline software | MacLean et al., 2009 | https://skyline.ms/project/home/software/Skyline/begin.view |
| ChemStation E.02.02.1431 | Agilent | https://www.agilent.com/en/product/software-informatics/analytical-software-suite |
| FluxFix: Isotopologue Analysis Tool v0.1 | Trefely et al., 2016 | http://fluxfix.science/http://fluxfix.science/ |
| Image Lab Software v6.1 | Bio-Rad |
https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z; RRID:SCR_014210 |
| Morpheus | Broad Institute | https://software.broadinstitute.org/morpheus |
| Harmony High-Content Imaging and Analysis Software v4.5 | PerkinElmer | https://www.perkinelmer.com/product/harmony-4-9-office-license-hh17000010 |
| ImageJ v1.53a | Schneider et al., 2012 | https://imagej.nih.gov/ij/index.html; RRID:SCR_003070 |
| FlowJo v10.7.2 | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads; RRID:SCR_008520 |
| Other | ||