|
Antibodies
|
| CRM1 antibody (XPO1); 1:1000 |
Abcam |
Cat# ab24189, RRID: AB 2257217 |
| SNF5 antibody [EPR6966]; 1:1000 |
Abcam |
Cat# ab126734, RRID: AB 11127897 |
| gamma H2A.X (pS139) antibody [9F3]; 1:1000 |
Abcam |
Cat# ab26350, RRID: AB 470861 |
| p53 (7F5) Rabbit mAb antibody; 1:1000 |
CST |
Cat# 2527, RRID: AB 10695803 |
| Rb (4H1) Mouse mAb antibody; 1:2000 |
CST |
Cat# 9309, RRID: AB 823629 |
| Phospho-Rb (Ser780) antibody; 1:1000 |
CST |
Cat# 9307, RRID: AB 330015 |
| p21 Waf1/Cip1 (12D1) antibody; 1:1000 |
CST |
Cat# 2947, RRID: AB 823586 |
| p27 Kip1 (D37H1) mAb antibody; 1:1000 |
CST |
Cat# 3688, RRID: AB 2077836 |
| Cleaved PARP (Asp214) (D64E10) antibody; 1:1000 |
CST |
Cat# 5625, RRID: AB 10699459 |
| c-Myc (D84C12) mAb antibody; 1:1000 |
CST |
Cat# 5605, RRID: AB 1903938 |
| Anti-β-Actin antibody; 1:5000 |
Sigma-Aldrich |
Cat# A5441, RRID: AB 476744 |
| Anti-α-Tubulin antibody; 1:1000 |
Sigma-Aldrich |
Cat# T9026, RRID: AB 477593 |
| IRDye 680RD Donkey anti-Mouse IgG antibody; 1:5000 |
LI-COR Biosciences |
Cat# 926-68072, RRID: AB 10953628 |
| IRDye 800CW Donkey anti-Rabbit IgG antibody; 1:15000 |
LI-COR Biosciences |
Cat# 926-32213, RRID: AB 621848 |
| Monoclonal Mouse Anti-Human Ki-67 Antigen; 1:150 |
Agilent |
Cat# M7240, RRID: AB 2142367 |
| Cleaved Caspase-3 (Asp175) antibody; 1:1000 |
CST |
Cat# 9661, RRID: AB 2341188 |
| Biotinylated horse anti-mouse IgG; 1:50 |
Vector Labs |
Cat# MKB-22258 |
| Biotinylated goat anti-rabbit; 1:50 |
Vector Labs |
Cat# PK6101 |
| Tyramide Alexa Fluor 568 |
Invitrogen |
Cat# T20949
|
| DAPI |
Sigma Aldrich |
Cat# D9542 |
| Mowiol |
Sigma Aldrich |
Cat# 81381 |
|
Chemicals, peptides, and recombinant proteins
|
| Dulbecco’s Modified Eagle Medium (DMEM) |
ThermoFisher Scientific |
Cat# 12491015 |
| DMEM/F-12 |
ThermoFisher Scientific |
Cat# 11320033 |
| Iscove’s Modification of DMEM (IMDM) |
Corning |
Cat# 10-016 |
| Fetal Bovine Serum (FBS) |
Corning |
Cat# 35-010 |
| Antibiotic-Antimycotic (100X) |
ThermoFisher Scientific |
Cat# 15240062 |
| L-Glutamine (200 mM) |
ThermoFisher Scientific |
Cat# 25030081 |
| Selinexor |
Selleckchem |
Cat# S7252 |
| Eltanexor |
Selleckchem |
Cat# S8397 |
| Ethanol |
Merck |
Cat# 100983 |
| Phosphate Buffered Saline |
Gibco |
Cat# 10010023 |
| Ribonuclease H (RNase H) |
Sigma-Aldrich |
Cat# 10786357001 |
| Propidium iodide |
Sigma-Aldrich |
Cat# P4170 |
| Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
Cat# D2650 |
| RIPA Buffer |
Sigma-Aldrich |
Cat# R0278 |
| Pierc BCA Protein Assay Kit |
Thermofisher Scientific |
Cat# 23225 |
| Formalin solution, neutral buffered, 10% |
Sigma-Aldrich |
Cat# HT501128 |
| EZ Prep Concentrate |
Ventana |
Cat# 950-102 |
| ULTRA Cell Conditioning (CC1) |
Ventana |
Cat# 950-224 |
| Background Buster |
Innovex |
Cat# NB306 |
|
Critical commercial assays
|
| CellTiter-Glo® Luminescent Cell Viability Assay |
Promega |
Cat# G7572 |
| Caspase-Glo® 3/7 Assay |
Promega |
Cat# G8091 |
| Nuclear Extraction Kit |
Abcam |
Cat# ab113474 |
| NuPAGE™ 4 to 12%, Bis-Tris, 1.0-1.5 mm, Mini Protein Gels |
Invitrogen |
Cat# NP0321BOX |
| QIAGEN QIAamp Tissue Kit |
Qiagen |
Cat# 51404 |
| RNeasy Micro Kit |
Qiagen |
Cat# 74004 |
| Agilent SureSelectXT All Exon V5 + UTRs |
Agilent |
Cat# 5191-7408 |
| TruSeq Stranded Total RNA LT Sample Prep Kit |
Illumina |
Cat# 20020596 |
|
Deposited data
|
| RNA-seq data from cell lines and used for perturbation screen |
Gene Expression Omnibus (GEO) |
GEO Accession: GSE198000
|
| Oncotarget predictions |
Figshare |
https://figshare.com/sZ3213cfaf6b2a2aeb7783
|
|
Experimental models: Cell lines
|
| 786-O |
ATCC |
Cat# CRL-1932 |
| BJ |
Dr. Alex Kentsis; MSKCC |
N/A |
| BT12 |
Dr. Alex Kentsis; MSKCC |
N/A |
| BT16 |
Dr. Alex Kentsis; MSKCC |
N/A |
| CHLA-266 |
COG cell line repository |
RRID: CVCL_M149 |
| COGW408 |
Dr. C. Patrick Reynolds; Texas Tech University |
N/A |
| G401 |
ATCC |
Cat# 87042204, RRID: CVCL 0270 |
| KP-MRT-AN |
Dr. Hajime Hosoi’s; Kyoto Prefectural University of Medicine |
N/A |
| KP-MRT-NS |
Dr. Hajime Hosoi’s; Kyoto Prefectural University of Medicine |
N/A |
| KP-MRT-YM |
Dr. Hajime Hosoi’s; Kyoto Prefectural University of Medicine |
N/A |
| RH-30 |
ATCC |
CVCL 0041 |
| RPE |
Dr. Alex Kentsis; MSKCC |
N/A |
| TC-71 |
ATCC |
CVCL 2213 |
| Wilms 1 |
Dr. Brigitte Royer-Pokora; Heinrich-Heine-University |
N/A |
| Wilms 10 |
Dr. Brigitte Royer-Pokora; Heinrich-Heine-University |
N/A |
| WiT49 |
Dr. Herman Yeger; University of Toronto |
N/A |
|
Experimental models: Organisms/strains
|
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
|
The Jackson Laboratory |
Cat# 005557; RRID: IMSR_JAX: 005557 |
|
Software and algorithms
|
| Prism Software Version 9 |
GraphPad |
N/A |
