Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-EspA-polyclonal, rabbit | UT Southwestern, Vanessa Sperandio | N/A |
| Anti-EspB-polyclonal, rabbit | UT Southwestern, Vanessa Sperandio | N/A |
| Anti-rabbit-HRP, goat | BioRad | Cat# 1706515 |
| Bacterial and Virus Strains | ||
| E. coli HS | Center for Vaccine Development at the University of Maryland School of Medicine | N/A |
| C. rodentium DBS100 | Tufts University, John Leong | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dulbecco eagle medium, low glucose | Invitrogen | Cat# 11885-084 |
| Cocaine Hydrochloride | Sigma-Aldrich | Cat#C5776 |
| LB agar, Miller | Fisher Scientific | Cat# BP1425 |
| LB broth, Miller | Fisher Scientific | Cat# BP9723 |
| Ampicillin powder | Sigma-Aldrich | Cat# A9518 |
| Naladixic acid power | Fisher Scientific | Cat# BP908 |
| L-(+)-Arabinose | Sigma-Aldrich | Cat# A3256 |
| Dextran sulfate sodium salt | MP Biomedicals | Cat#021601109 |
| Phusion High fidelity PCR master mix | Thermo Fisher | Cat# F-531L |
| Random primers | Invitrogen | Cat# 48190-011 |
| Super script II reverse transcriptase | Invitrogen | Cat# 18064-014 |
| Sybr green master mix | Thermo Fisher | Cat# 4309155 |
| Sarcosine | Sigma-Aldrich | Cat#131776 |
| Glycine Hydrochloride | Sigma-Aldrich | Cat# G2879 |
| Trizol reagent | Thermo Fisher | Cat# 1559618 |
| Chloroform | IBM Scientific | Cat# 67-66-3 |
| dNTP mix 10mM | Invitrogen | Cat# 18-427-088 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# A2153 |
| Dopamine Hydrochloride | Sigma-Aldrich | Cat# H8502 |
| 6-hydroxydopamine Hydrochloride | Sigma-Aldrich | Cat# H4381 |
| RNAlater-ICE | Fisher Scientific | Cat# 44-275-75 |
| Protease inhibitor cocktail | Sigma-Aldrich | Cat# P8849-5 |
| Dithiothreitol (DTT) | Fisher Scientific | Cat# BP172-25 |
| Formaldehyde | Fisher Scientific | Cat# BP539 |
| Brain heart infusion agar | Becton Dickinson | Cat# 2418102 |
| Dextran sulfate sodium salt | Fisher Scientific | Cat# 02-160-1109 |
| Metronidazole | MP biomedicals | Cat# 0215571025 |
| Neomycin trisulfate salt hydrate | Sigma-Aldrich | Cat# N5285 |
| Vancomycin hydrochloride | Research Products International | Cat# V06500 |
| Critical Commercial Assays | ||
| RNeasy Power Microbiome | Qiagen | Cat# 26000-50 |
| RNeasy Plus Mini Kit | Qiagen | Cat# 74134 |
| RiboPure RNA isolation Kit | Ambion | Cat# 1405072 |
| GenElute Plasmid Miniprep | Sigma | Cat# PLN350-1KT |
| QIAquick PCR purification kit | Qiagen | Cat# 28906 |
| Glycine Assay Kit | Sigma-Aldrich | Cat# MAK261 |
| Deposited Data | ||
| RNA sequencing data | https://www.ncbi.nlm.nih.gov/geo/ | GSE212421 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J mice | The Jackson laboratory | Cat# 000664 |
| Oligonucleotides | ||
| Primers for qRT-PCR used in this study are listed in Table S1 | This paper | N/A |
| Software and Algorithms | ||
| Prism 6 | GraphPad | N/A |
| QuantStudio real-time PCR software v1.3 | Thermo Fisher | N/A |
| Image Lab 5.2.1 | BioRad | N/A |
| iDEP.91 | http://bioinformatics.sdstate.edu/idep/ | N/A |
| CLC Bio microbial genomics module | https://www.qiagenbioinformatics.com/plugins/clc-microbial-genomics-module/ | N/A |
| MetaboAnalyst 5.0 | https://www.metaboanalyst.ca/ | N/A |
| Fiji Image J | https://fiji.sc/ | N/A |
| Fusion 6.5v | Omnitech Electronics Incorporated | N/A |
| MultiQuant 3.0.3. Software | https://sciex.com/products/software/multiquant-software | N/A |