Skip to main content
. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Nat Biomed Eng. 2022 Jul 14;6(11):1298–1316. doi: 10.1038/s41551-022-00909-y

Fig. 2 |. AAV serotypes selectively target pancreatic autonomic efferent and afferent nerves.

Fig. 2 |

a, Confocal images of iDISCO+ cleared CG, NG and DRG demonstrating mCherry+ pancreas-innervating neurons 4 weeks after intrapancreatic injection of AAV-hSyn-mCherry (top to bottom: serotypes 9, 8, 6 and rAAV2-retro (AAVrg)). Scale bars, 100 μm. N = 4 biologically independent samples. b, Confocal images of iDISCO+ cleared pancreas demonstrating mCherry+ neurons within intrapancreatic ganglia stained for NF200, 4 weeks after intrapancreatic injection of AAV-hSyn-mCherry. N = 4 biologically independent samples. Scale bars, 50 μm. c, Quantification of mCherry+ pancreas-innervating neurons in CG (top), NG (middle) and DRG (bottom), 4 weeks after intrapancreatic injection of AAV-hSyn-mCherry (left: percentage of total neurons per ganglion; right: total number per ganglion). d, Quantification of mCherry+ pancreas-innervating neurons in intrapancreatic ganglia, 4 weeks after intrapancreatic injection of AAV-hSyn-mCherry (mCherry volume as percentage of NF200+ volume of the ganglia). All data represented as mean ± SEM. e, Confocal image demonstrating specific neural expression of AAV8-hSyn-mCherry in intrapancreatic ganglia immunolabelled with NF200 with adjacent islet of Langerhans. Top left, mCherry (red) and insulin (green); top right, mCherry (red) and NF200 (blue); bottom left, NF200 (blue) and insulin (green); bottom right, mCherry (red), NF200 (blue) and insulin (green). Scale bars, 50 μm. Biologically independent samples: c, left and right: for CG n = 11 (AAV9), 6 (AAV8), 9 (AAV6), 4 (AAVrg); for NG n = 7 (AAV9), 9 (AAV8), 6 (AAV6), 8 (AAVrg); and for DRG: n = 16 (AAV9), 7 (AAV8), 8 (AAV6), 11 (AAVrg); d, n = 4 animals per group. Statistical analyses are described in Supplementary Table 2.