Figure 5.

Transplantation of mutant IMPG2 hPSC-ROs into the subretinal space of immunocompromised rats restores their capacity to extend and maintain OS

(A) Schematic summarizing the experimental approach used to transplant day 45 hPSC-ROs into the subretinal space of Foxn1-S334ter immunocompromised rats, a time point when this model is nearly devoid of host photoreceptors.

(B–H) Immunocytochemical analysis of human donor photoreceptor rosettes 6 months after RO transplantation reveals extensive RHO+/PHPR2+ OS in all mutant and control lines: IMPG2^{Y254C/A805(fs)ter} (B), IMPG2^Y254C/GC (C), IMPG2^KO/KO (D), IMPG2^GC/GC (E), WA09-IMPG2^+/+ (F), WA09-IMPG2^Y254C/Y254C (G), and WA09-IMPG2^KO/KO (H). Scale bars, 25 μm.