Figure 2.

The HSC marker expression in iHSCs compared with pHSCs

(A) qRT-PCR analysis of gene expression of HSC markers (ALCAM, PCDH7, GFAP, PDGFRβ, Desmin, hSTAP, and PDGFRα), a fibroblast marker (THY-1), activated HSC markers (COL1α1, COL3α1, and ACTA2), and a hepatic marker (AFP); n = 3 in each group.

(B) Representative images of the markers GFAP, PCDH7, NCAM, nestin, α-SMA, collagen I, and desmin in iHSCs, pHSCs (p0 and p5), and fibroblast cells. Scale bars, 100 μm.

(C) Representative images of the iHSC morphology at different passages of iHSCs and pHSCs. Scale bars, 100 μm.

(D) qRT-PCR analysis of gene expression in iHSCs at different passages. Data are shown as the mean ± SEM from three independent experiments; n = 3 in each group. Student’s t test was used. ^∗p < 0.05.