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. 2022 Nov 3;13:996166. doi: 10.3389/fphys.2022.996166

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Copyright © 2022 Xie, Zhen, Liu, Chen, Liu, Zhou, Zhou, Hu, Zhu, Huang, Zhang and Nie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Medicinal charcoal tablets inhibit the changes of cardiac hypertrophy markers and SERCA2 expression in 5/6 Nx mice on a high-choline diet. (A-D) The mRNA levels of ANP, BNP, β-MHC, and α-MHC were measured by real-time PCR. (E) The protein levels of SERCA2 were measured by western blotting. (F) Graphic representation of relative abundance of SERCA2 normalized to GAPDH. (G) The mRNA levels of SERCA2 were measured by real-time PCR. The data are presented as the mean ± SD. ^* p < 0.05 and ^** p < 0.01 vs. CKD + Choline group. n = 6 in each group.