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Filterable soluble factors released by T. cruzi inhibit the basal production of IL-12 (p40) and TNF-α. DC were cultured in the bottom of wells and separated by a cell-impermeable filter from trypomastigotes placed in a transwell. The parasite-to-cell ratios were 5:1 and 10:1. After 24 h, supernatants were collected to determine the cytokine production by ELISA. Results of one representative experiment of three are shown.
