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T. cruzi infection and TCM inhibit LPS-induced IL-12 (p70) release from DC. DC were stimulated with LPS (10 ng/ml) in the presence of trypomastigotes at different parasite-to-cell ratios (A) or in the presence of increasing concentrations of TCM (B). After 24 h, supernatants were collected for determination of IL-12 (p70) levels by ELISA. Results of one representative experiment of two are shown.
