FIG. 6.

T. cruzi infection and TCM inhibit LPS-induced HLA-DR and CD40 up-regulation. DC were incubated with LPS (10 ng/ml) with or without trypomastigotes (parasite-to-cell ratio, 30:1) (A) or TCM (75%) (B). DC incubated in the presence of medium alone were used as control. After 24 h, DC were harvested and washed, and the expression of HLA-DR (left) and CD40 (right) was determined by flow cytometry. Thin lines represent FACS profiles obtained for DC incubated with medium alone. Thick lines represent FACS profiles obtained for DC incubated with LPS alone. Dotted lines represent FACS profiles obtained for DC incubated with LPS in the presence of trypomastigotes (A) or TCM (B). The solid histograms represent FACS profiles after staining with the corresponding isotype-matched control MAb. Results of one representative experiment of six are shown.