TABLE 2.
DC viability is not affected by TCMa
| TCM concn (%) | % of cells labeledb by:
|
||
|---|---|---|---|
| Annexin V | Annexin V and PI | PI | |
| 0 (control) | 4 ± 2 | 7 ± 2 | 17 ± 5 |
| 12.5 | 3 ± 1 | 8 ± 1 | 23 ± 5 |
| 25 | 3 ± 1 | 10 ± 2 | 23 ± 6 |
| 50 | 3 ± 1 | 11 ± 3 | 22 ± 3 |
| 75 | 3 ± 1 | 9 ± 2 | 22 ± 4 |
a
DC were incubated for 24 h in medium alone (control) or in the presence of TCM at different concentrations. They were then washed and incubated with annexin V-FITC followed by PI, and staining was analyzed by flow cytometry. Annexin V staining corresponds to early apoptosis, Annexin V PI staining corresponds to late apoptosis, and PI staining alone corresponds to necrotic cells.
b
Data represent the percentage of positive cells (mean ± standard error of the mean) in each quadrant of dot plot analysis in four independent experiments with four different blood donors.