TABLE 3.

TCM inhibits LPS-induced HLA-DR and CD40 up-regulation^a

Condition(s)	Surface molecule expression (MFI)b
	HLA-DR	CD40	CD54	CD80	CD86
Medium	216 ± 3	55 ± 4	84 ± 31	21 ± 5	33 ± 16
Medium + TCM	232 ± 41	46 ± 4	55 ± 19	19 ± 5	33 ± 16
LPS	1,006 ± 419	99 ± 11	125 ± 35	45 ± 6	106 ± 20
LPS + TCM	680 ± 244*	72 ± 9*	114 ± 26	35 ± 4	72 ± 4

^aDC were stimulated with LPS (10 ng/ml) in the presence or absence of TCM (75%). After 2 h, the DC were harvested and washed, and the expression of surface molecules was determined by flow cytometry analysis. As a control, DC were incubated in medium alone in the presence or the absence of TCM (75%). 

^bResults are expressed as median fluorescence intensity (MFI) and represent the mean ± standard error of the mean of six independent experiments with six different donors. Cell labeling with control MAb was not affected by TCM (data not shown). *, P ≤ 0.01 by nonparametric Wilcoxon’s paired one-tailed test, compared to MFI in the absence of TCM.