TABLE 1.
Approaches for the detection of m6A RNA methylation.
| Approaches | Principle | Advantages | Limitations | References |
|---|---|---|---|---|
| m6A dot blot | Antibody based immunoblot | Easy, fast | Low sensitive, semi-quantitative | Nagarajan et al. (2019) |
| HPLC-MS/MS | Mass spectrum | High sensitive, quantitative | Lack of RNA sequence and localization | Thuring et al. (2017) |
| SCARLET | Thin-layer chromatography | Quantitative | Complicated, low-throughput | Liu and Pan, (2016) |
| m6A-Seq | Antibody based sequencing | High-throughput | 100–200 nucleotide resolution | Dominissini et al. (2012) |
| MeRIPSeq | Antibody based sequencing | High-throughput | 100–200 nucleotide resolution, antibody specificity | Meyer et al. (2012) |
| miCLIP | Antibody based sequencing | High-throughput, single site | Low cross-linking efficiency, antibody specificity | Linder et al. (2015) |
| MAZTER-Seq | Endoribonuclease based sequencing | High-throughput, single site | Preference of the enzyme | Garcia-Campos et al. (2019) |
| m6A-REF-Seq | Endoribonuclease based sequencing | High-throughput, single site | Preference of the enzyme | Zhang Z. et al. (2019) |
| m6A-SEAL | Chemical labeling sequencing | High-throughput, single site | Chemical labeling efficiency | Wang Y. et al. (2020) |
| m6A-label-seq | Chemical labeling sequencing | High-throughput, single site | Chemical labeling efficiency | Shu et al. (2020) |
| m6A-SAC-seq | Chemical labeling sequencing | High-throughput, single site | Chemical labeling efficiency | Hu et al. (2022) |