TABLE 3.
The characteristics of stool donor.
| Author, year | Number of donors | Sex | Exclusion criteria of donors | Donor standardization |
Factor that positively influence microbiome diversity |
||||||||||
| Maintain long term behavior | Stability of microbiome composition over time | Specific characteristics |
Microbiome diversity | ||||||||||||
| Normal BMI | Age < 50 year | Born vaginally | Non-smoker | Regular exercise | Without frequent treatment with antibiotics | Consuming a sport-specific diet | Without first degree relative | ||||||||
| Well-defined donor | (45, 17) | 1 | Male | Yes | Prohibited from taking medication | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | A significant degree of microbial diversity (normobiotic) with bacterial signature including an abundance of Streptococcus, Dorea, Lactobacillus, and Ruminococcaceae spp. These four genera of bacteria have been reported to constitute favorable bacteria for a donor. |
| (45, 17) | 1 | Male | Yes | Prohibited from taking medication | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | A significant degree of microbial diversity (normobiotic) with bacterial signature including an abundance of Streptococcus, Dorea, Lactobacillus, and Ruminococcaceae spp. These four genera of bacteria have been reported to constitute favorable bacteria for a donor. | |
| (18, 18) | 2 | Male | Yes | Prohibited from taking medication | Yes | Yes | Yes | N/A | N/A | N/A | N/A | N/A | N/A | Two donors had high microbial diversity | |
| and travelling abroad. Were requested to maintain a stable diet during the study. | |||||||||||||||
| Relatively well-defined donor | (20, 20) | 4 | N/A | Yes | Informed to maintain a healthy lifestyle throughout the collection period | N/A | Yes | Yes | Yes | Yes | N/A | N/A | N/A | N/A | Donors had higher microbiome biodiversity than patients with IBS |
| (38, 38) | 2 | N/A | Yes | Asked to keep their medication and diet stable over the entire study period. | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | The subject with the highest abundance of the butyryl-CoA CoA transferase gene in their fecal sample was selected as donor. | |
| (39, 39) | 1 | Male | Yes | Prohibited from taking medication and traveling abroad | N/A | Yes | Yes | Yes | Yes | N/A | Yes | N/A | N/A | Donors had higher microbiome richness than patients with IBS | |
| Unclearly defined donor | (21, 21) | 4 | N/A | N/A | N/A | N/A | Yes | Yes | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| (21, 21) | 4 | N/A | N/A | N/A | N/A | Yes | Yes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | |
| (19) | 2 | N/A | Yes | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | |
N/A. Not available; BMI, Body mass index.