Figure 2.

Characterization of transgene expression in Nestin-GFPcyt and Nestin-CFPnuc transgenic spinal cords

(A–L) Double immunohistochemistry for GFP (A–F) and CFP (G–L) and cell-specific markers in the spinal cord parenchyma (A–C and G–I) or central canal (D–F and J–L): GFAP⁺ astrocytes (A, D, G, and J), IBA1⁺ microglia (B, E, H, and K), and NG2⁺ oligodendrocyte progenitors (C, inset a, and I, inset a) or pericytes (C, inset b; I, inset b; and L). Boxed areas are enlarged in the right panels.

(M and N) Visualization by EM of immunogold-labeled ependymal cell nuclei in the Nestin-CFPnuc spinal cord. E, ependymal cell.

Scale bars: 50 μm (A–L), 5 μm (M and N), and 1 μm (E2).