Figure 5.

Fate mapping of adult DRG SCs

(A) Schematic representation of tamoxifen induction and temporal analysis of Nestin-CreER^T2/Rosa26-YFP mice.

(B and C) Detection of adult DRG-YFP⁺ cells 2 and 90 days after tamoxifen induction (dpi).

(D) Number of DRG transgenic positive cells per square millimeter at 2 dpi in CFPnuc mice and ipsi/contra injured or non-injured mice.

(E) Temporal evolution of the number of DRG-YFP⁺ cells per square millimeter.

(F and G) Immunodetection of adult DRG-YFP⁺ cells with SOX10 (glial cells) and NEU-N (neurons) at 2 (F) and 90 dpi (G) in non-injured Nestin-CreER^T2/Rosa26-YFP mice. Boxed areas are enlarged in the right panels.

Data are shown as mean ± SEM (n = 3). One-way ANOVA and multiple comparisons: Dunnett’s test (D) and Tukey’s test (E); ^∗∗p < 0.01, ^∗p < 0.05. See also Figure S5.