Figure 7.
Long-term lineage tracing of adult DRG SCs in axotomized Nestin-CreERT2/Rosa26-YFP animals
(A–F) Immunostaining of contra versus ipsi DRGs for YFP at 2, 15, and 60 days after axotomy.
(G) Temporal evolution of the DRG-YFP+ cell density ratio (ipsi over contra side).
(H–K) Immunocharacterization of adult DRG-YFP+ cells using SOX10 for glial cells (H and J) and NEU-N for neurons (I and K) in the contra or ipsi DRG at 90 dpi.
Scale bar: 50 μm (A–F and H–K). Boxed areas are enlarged in the right panels. In (G), data are shown as mean ± SEM, (n = 3). Two-way ANOVA and multiple-comparisons Dunnett’s test; ∗p < 0.05. See also Figure S6.