FIGURE 1.

Effect of osmotic shock injury on Ca²⁺ transients in FDB fibers preloaded with different concentrations of BAPTA-AM. A/J or A/JCr myofibers were loaded with Rhod-2AM, with or without additional BAPTA-AM, subjected to 1 Hz stimulation, and imaged in line-scan mode under confocal optics (see Methods). Representative line-scan confocal images of voltage-induced Ca²⁺ transients in A/JCr (A) and sham (vehicle only) A/J [(B): A/J] fibers and in A/J fibers preloaded with BAPTA-AM at 5, 10, 50 and 100 nM (C). All examples in (B,C) were exposed to equal amounts of DMSO (1.5% by volume). All examples are shown before osmotic shock injury (OSI) and 5 min after OSI. Bars, 100 µm (vertical) and 250 ms (horizontal).