FIGURE 4.

Effect of osmotic shock injury on Ca²⁺ transients in A/J FDB fibers preloaded with different concentrations of EGTA-AM. As in Figure 2, but with myofibers loaded with EGTA-AM. (A), averaged amplitudes of Ca²⁺ release before OSI presented as (F_max-F₀)/F₀. (B), averaged data for recovery of Ca²⁺ transients at 5 min after OSI. (C), averaged data for frequency of Ca²⁺ waves at 5 min after OSI. (D), % fibers that produced Ca²⁺ waves at 5 min after OSI. Dashed lines represent values obtained with A/JCr fibers. *, p < 0.05 compared to A/J. N is indicated in each bar. Student’s t test was used for (A–C); Χ ² was used for (D)