FIGURE 5.

Distributions of GCaMP6f_u and GCaMP6f_u-DYSF-ΔC2A in sarcoplasm. (A). Cartoon diagrams of chimeric structures, which include the CMV promoter, the GCaMP6f_u reporter, and, for GCaMP6f_u-DYSF-ΔC2A, the dysferlin ORF excluding most of the C2A domain (i.e., residues 108–2080) but including the remaining C2 domains B thought G (green hexagons), the Fer and DysF domains in the middle of the molecule (blue and pink outlined rectangles) and the transmembrane domain (blue rectangle near the C terminus; see Methods). (B). Subcellular distribution of Venus-GCaMP6f_u and GCaMP6f_u-DYSF-ΔC2A in transfected A/J myofibers. Plasmids were electroporated into A/J myofibers and imaged under confocal optics 2 weeks later. Double arrows: transverse tubules at level of triad junctions, as reported (Kerr et al., 2013; Muriel et al., 2022); single arrows: Z-disks, as reported (Muriel et al., 2022). Bars, 10 µm.