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FAF1–Hsp70 interaction is crucial for FAF1 regulation of RhoA activation. HeLa cells with FAF1 knockdown were transfected with FAF1 WT and FAF1H160A mutant. Then, RhoA activation (A) and MLC2 phosphorylation (B) levels were measured, and actin stress fiber formation was detected by confocal microscopy (C). Scale bar, 10 μm.
