Figure 7.

FAF1 inhibits cancer cell invasion in MDA-MB-231 cells by regulating the integrity of adherens junction. (A and B) MDA-MB-231 cells were transfected with control siRNA or FAF1 siRNA for 48 h and then spheroid formation was initiated in 2.5% Matrigel. Then, size kinetics were measured by calculating volume changes based on the radius of each spheroid. Representative images (A) and relative volume of the 3D spheroid cell invasion (B) are shown. Error bars represent standard deviation (n = 3). Scale bar, 200 μm. (C) Schematic diagram of the effect of FAF1 on stabilizing adherens junction by suppressing RhoA activation.