Table 1.

Statistics on the data collection and refinement.

	FAF1 UBL1				FAF1 L-UBL1 complexed to
					Hsp70 NBD with ADP + Pi
	Native	SeMet MAD			Native
		Peak	Edge	Remote
Data collection
Beam line	PLS BL-5C
Wavelength, Å	1.0000	0.9794	0.9796	0.9717	1.0000
Space group	P42212				P3121
Cell dimensions
a, Å	65.02	65.02			103.33
b, Å	65.02	65.02			103.33
c, Å	33.56	33.55			127.09
α, °	90.00	90.00			90.00
β, °	90.00	90.00			90.00
γ, °	90.00	90.00			120.00
a Resolution, Å	50.0–1.2	50.0–1.6			50.0–2.2
	(1.28–1.20)	(1.66–1.60)			(2.28–2.20)
Total reflections	659957	321179	321309	32847	1609110
Unique reflections	23153	18815	18830	18813	40388
a Completeness, %	99.7 (99.6)	99.3 (99.0)	99.2 (98.8)	99.2 (98.8)	99.9 (99.9)
a I/σ(I)	49.6 (6.9)	37.9 (6.8)	37.3 (6.9)	37.8 (7.1)	24.4 (5.2)
b R merge, %	5.0 (21.6)	8.3 (20.7)	6.5 (19.6)	6.4 (19.7)	9.8 (32.2)
Refinement
Resolution, Å	50.0–1.2				50.0–2.2
c R cryst/Rfree, %	22.2/24.3				17.4/20.6
No. of protein atoms	591				3638
No. of water atoms	169				454
RMS deviation
Bond length, Å	0.005				0.007
Bond angle, °	0.771				0.876
Ramachandran analysis
Favored region, %	98.63				96.92
Allowed region, %	1.37				3.08
PDB code	7FGN				7FGM

^a Values in parentheses are for the outer most resolution shell.

^b R _merge = ∑_h∑_i|I(h,i)−<I(h)>|/∑_h∑_iI(h,i), where I(h,i) is the intensity of the i^th measurement of reflection h and <I(h)> is the mean value of I(h,i) for all i measurements.

^c R _free was calculated from randomly selected 5% set of reflections not included in the calculation of the R value.