FIGURE 6.

Single-Cell RNA sequencing of the injured mouse brain. A total of 23 unique cell clusters were identified (A), with similar cellular composition and RNA feature counts between shams and rmTBI mice (B,C). In total, 5,684 pathways were significantly enriched in neuronal cell types (D), of which 636 (11.2%) were uniquely enriched in a single cluster (E). Gene set enrichment analysis (GSEA) revealed a senescence-like expression profile in neuronal clusters (F) including DNA damage and cell cycle control, senescence-associated inflammation, senescence-associated anti-apoptotic mechanisms, and cell specific changes with potential functional repercussions. Glial cells shared a total of 161 enriched pathways alongside unique features for each cell type (G). GSEA revealed a similar expression profile in glial cells to neurons, indicating senescence and cell-specific functional changes (H).